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Protein extract from the cells to how the proteins are present in the cell and have the discussion on that
Typology: Summaries
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Protein extraction and SDS-Page Merck Innovation Lab, Bangalore Uchenna W, Ph.D. 26 th^ April 2023
Contents https://www.uihere.com/
https://www.intechopen.com/ Expression
Proteins Genome - The Instruction Manual of Life Protein: Key Molecule
Sickle Cell Disease
https://link.springer.com/article/10.1007/s00277-019-03813-9 https://www.aboutkidshealth.ca/Article?contentid=745&language=English Levels of Protein Structure
https://pdb101.rcsb.org/global-health/diabetes-mellitus/drugs/insulin/insulin
https://commons.wikimedia.org/wiki/File:Protein_primary_structure.svg https://www.quora.com/What-are-the-levels-of-a-protein-structure Protein Primary Structure Protein Secondary Structure
Image credit: OpenStax Biology
Levels of Protein Structures
https://alevelnotes.com/notes/biology/biological-molecules/biological-molecules/protein-structure Types of Interactions
1 2 3 4 Therapeutics Diagnosis^ Biomarkers^ R & D Protein based drugs Antibodies Insulin Cytokines Teriparatide Hormones levels Renal disease Cancer Diabetes Protein Biomarkers PSA Her- Amyloid beta α-synuclein Protein-protein interaction Protein function Protein structure Importance of studying proteins Protein Extraction https://www.sonomechanics.com/cell-disruption/ https://www.thermofisher.com/
Tracking dye Migrate with the leading edge of the proteins being separated Increases density of sample Allow the samples to sink into each well Reduce disulfide ridges between cysteines Denatures, linearizes the proteins, coat them with negative charge Loading buffer Protein Separation
SDS-Polyacrylamide Gel Reagent Function Acrylamide Forms linear polymers Bis-acrylamide create crosslinks between acrylamide Tris HCl Buffer (7-9 pH range) 10% SDS Denature the protein 10% APS oxidizing agent (produce free radicals) TEMED Catalyze the polymerization β-ME Reduces the di-sulfide bonds
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