Classify microbes into 5 groups on the basis of preferred temperature range
Indentify how and why the pH of culture media is controlled
Explain the importance of osmotic pressure to microbial growth
Name a use for each of the 4 elements needed in large amounts for microbial growth
Explain how microbes are classified on the basis of oxygen requirements
Identify ways in which aerobes avoid damage by toxic forms of oxygen
Describe the formation of biofilms and their potential for causing infection
Distinguish chemically defined and complex media
Justify the use of each of the following: anaerobic techniques, living host cells, candle jars,
selective and differential media, and enrichment medium
Differentiate biosafety levels 1,2,3,&4
Define colony
Describe how pure cultures can be isolated by using the streak plate method
Explain how microorganisms are preserved by deep-freezing and lyophilization (freeze-drying)
Define bacterial growth, including binary fission
Compare the phases of microbial growth and describe their relation to generation time
Define: sterilization, disinfection, antisepsis, degerming, sanitization, biocide, germicide,
bacteriostasis, & asepsis
Describe the patterns of microbial death caused by treatments with microbial control agents
Describe the effects of microbial control agents on cellular structures
Compare the effectiveness of moist heat (boiling, autoclaving, pasteurization) and dry heat
Describe how filtration, low temps, high pressure, desiccation, & osmotic pressure suppress
microbial growth
Explain how radiation kills cells
List the factors related to effective disinfection
Interpret the results of use-dilution tests and the disk-diffusion method
Identify the methods of action and preferred uses of chemical disinfectants
Differentiate halogens used as antiseptics from halogens used as disinfectants
Identify the appropriate uses for surface-active agents
List the advantages of glutaraldehyde over other chemical disinfectants
Identify chemical sterilizers
Explain how the type of microbe affects the control of microbial growth
