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Steps to Successful Data Acquisition, Bio 112
Jeffrey Muday, Department of Biology
Preparation steps
- Verify hardware configuration and make sure power is turned on to every device.
- Verify software is installed correctly – does AcqKnowledge start without issuing an error message?
- Create a template file (this is detailed on another handout). It should be called “frogtemp.acq” These dialogs are set via the MP100 drop-down menu.
Figure: Setting up the channels and acquisition parameters
Acquisition steps
- Start the Biopac AcqKnowledge
- Prepare your experimental subject
- Begin data capture: use Alt+space on the keyboard to begin the acquisition
- Stop data capture: use Alt+space on the keyboard to stop the acquisition.
- SAVE YOUR DATA. Use a “meaningful” filename, but remember that filenames are limited to 8 characters only with NO SPACES.
- Scale your data: Automatic scaling – Horizontally Auto-scale first!
Vertically Auto-scale channel 1 (stimulator). Now, click on the channel number first, then click on the vertical auto-scale button.
Vertically Auto-scale channel 2 (force)
Figure: Measuring the latency period
With the I-Beam cursor, you can highlight a slice of time from your acquisition graph. To highlight, move your cursor to the starting point, hold down on the left button and drag the mouse to the ending point. The measurement bar will present the selected function. The above illustration presents a student measuring the latency period of a frog gastroc muscle. In this experiment, it was (delta-T) 26 milliseconds.
- Measuring Latency period : highlight the area of latency, and read the measurement from the delta-T box.
- Measuring Contraction period : similar to measuring the latency, highlight the period of contraction and read the measurement from the delta-T box.
- Measuring Relaxation period : similar to measuring the latency, highlight the period of relaxation and read the measurement from the delta-T box.
- Measuring Amplitude of the contraction is slightly more difficult. You must change one of the measurement toolbar boxes to be CHANNEL 2 (Force) and use P-P measure:
Measuring Amplitude : highlight from a point of relaxation to just past the maximum contraction point. Read the measurement from the P-P box. The reading is in volts.
- Spatial and Temporal Summation, Tetanus and Fatigue
Figure: practical demonstration of Summation using AcqKnowledge.
