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Study guide for micro, Study notes of Microbiology

Use this as your micro study guide for quiz 3

Typology: Study notes

2024/2025

Uploaded on 04/18/2025

elia-gorelik
elia-gorelik 🇺🇸

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Microbiology Ch 78
Genome completesetofgenetic info
cells
mustundergo
DNA
replication
transcription
translation tomultiplyDivide
NAdeoxy
ribonucleicacid deoxyribose
phosphate
nucleobase
tyingfittings 5Pa
b
hate 3
Yarbon
VRNAribonucleicacidriboseuracil
RNAmessengerRNA
RNA ribosomal
RNA
TRNAtransferRNA
Keplication
forkstheres2ateach
origin yshaped forks
meetatterminating
sitewhencomplet
replication
startsatthe
originofreplication
Gyrase breaks
DNA
strand
helicase unwinds
DNA
helix
primaseenzyme
thatsynthesizes
RNA
sequencescalled
primers
DNAReplication
1Atthe
originhelicase
unwinds
DNA
2SSB
Proteins
bind
tothe
DNA
strandstokeepthem
aph case so primate polymerase Ligase
3primase
makes
RNA
primers
on
both
strands
4DNA
polymerase
builds
new
strands 5to3
5Ligase
takes
care
ofthegaps
betweenokazaki
fragments sealingthem together
DNAtranscription occurs innucleus
1MRNA
can
go
out
of
the
nucleusinto
cytoplasm
whereitattachesto aribosome ribosomes
makeprotein
2ribosomes
madeofrRNAbuilds
proteinfor
translation mrna n'Biome translation
The
MRNAinprokaryotescanbe
one
geneormultiple
Translation
1tRNA
carry
amino
acids
on
them
and
act
upon
the
signal
provided
bymRNA forth
aIdle
Phi
2tRNA
reads
thebasescodon
at
a
time
and
will
providethecomplimentarybase
pair
Sigma
factor recognizespromoter
Quorum
sensing cell
signaling
membrane spanningsensorchanges
internal
region phosphorylates Amino
Acids
responseregulator
turns
genesonoff
phosphate
group
transferred
from
sensor
antigenicvariation strategy
for
pathogensto
evade
bychanging
their
surfaceantigensso
theyreunrecogniza
phase
variation switches
geneson off
operon setof
regulated
genestranscribedas
MRNA
regulon separate
operonscontrolled
byasingleregulatorymechanism
pf2

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Microbiology

Ch

Genome

complete

setofgenetic info

cells

must

undergoDNA

replication

transcription translation to

multiply

Divide

NA

deoxy

ribonucleic

acid

deoxyribose phosphate

nucleobase

tying fittings

5Pa

b

hate

Yarbon

VRNA

ribonucleic acid riboseuracil

RNAmessenger

RNA

RNA

ribosomal RNA

TRNA

transferRNA

Keplication

forks

theres 2 at

each

origin

y

shaped

forks

meetat terminating

site

whencomplet

replication

starts

at

the

origin

of

replication

Gyrase breaks

DNA

strand

helicase

unwindsDNA

helix

primaseenzymethat

synthesizesRNA sequences

called

primers

DNAReplication

At

theorigin

helicase

unwindsDNA

SSB

Proteins

bindtothe

DNA

strands

to

keep

them

aph

case so

primate polymerase

Ligase

3

primase

makes

RNAprimerson

both

strands

4

DNA

polymerase

builds

new

strands 5 to

Ligase

takescareofthe

gaps

between okazakifragments sealing

them

together

DNAtranscription

occurs in

nucleus

1 MRNA

can

go

out

ofthe

nucleus

into

cytoplasm

where it

attaches

to a

ribosome

ribosomes make protein

2

ribosomesmade

ofrRNAbuilds

protein

for

translation

mrna

n'Biome

translation

The

MRNAinprokaryotes

canbeone

gene

or

multiple

Translation

1 tRNA carryamino

acids on them

and

actupon

the

signal provided

by

mRNA

forth

a

Idle

Phi

tRNA

reads

the

bases

codon

at

a

time andwill

provide the

complimentary base

pair

Sigma

factor recognizes

promoter

Quorum sensing

cell

signaling

membrane

spanning

sensorchanges

internal

region

phosphorylates Amino

Acids

response regulator

turns genesonoff

phosphate

group

transferred

from

sensor

antigenic

variation

strategy

for

pathogens

to

evade

by

changing their

surface

antigens

so

theyre

unrecogniza

phase

variation

switches genes

on off

operon setof

regulated genes

transcribed

as

MRNA

regulon separate

operons

controlled

by

a single

regulatory

mechanism

Constituitive

enzymes

constantlysynthesized

Inducibleenzymes

not

routinley

produced

made

only

when

needed

repressible enzymes constantly

produced

turned

off when

not

needed

2

most

common

waysto

control transcription

alternative

sigma

factor

or

standard

Sigma

factor

DNA

Binding

protein

canactas

repressors

or

activators

Induction

bindstooperator

blockstranscription

repression

cant

bindoperator

nor

blocktranscription without

co

repressor

sensing

glucose

levels

throughtransporter

Ilac

operon hasan

activatorcalled catabolite activator

protein

2 CAPbinds

activator

binding

site

and

is

required

for

transcription

CAP

functions

when

bound

by

cAMPcAMP madewhen

glucose

islow

auxotroph

deletion of gene

environmentdoesntcause

mutation

base

substitution

incorrectnucleotideincorporated

during DNA

synthesis

leads

to

silent

missensenonsense

mutations

frameshift mutation premature

stop

codon

transposons

jumping

genes

transposition

intercatalating agents

cause frameshift

mutations

Base

excision repair

DNA

glycosylase

removesdamaged

nucleobase

2 main

approaches

to

isolate

mutagens Direct

indirect

selection

ames test

measures effect

of

chemical

on

reversion

rate

Genes

are

transferred through

transformationBY

a

transduction

Yaffa

conjugationcells

transformation

uptakeof

naked DNA

transduction

transfer

of

bacterial

genes

by

bacteriophages

Conjugation DNA transfer

betweenbacterialcells

R

plasmids

encodeantibiotic

resistance

CRISPR

systems

clusters of

regularly

interspersed

short

palindromic

repeats

Vivon

complete

viralparticle

outside host

nucleicacidprotein

coat

enveloped

viruslipidbilayerenvelope

from

host

non

enveloped

virus

lacks

envelope more

resistant

to

disinfectants

lytic

phage

infectionswhen

it

exits

host cell

lyses

temperate

phage

infection infected

cellislysogen

lysogeny

consequences immune to

superinfection

infected

by

same

phage

know

Hep

B vs

Hep

C