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Name __ Any Date Period Uv Lab: Using a Compound Light Microscope Background: Microscopes are very important tools in biology. The term microscope can be translated as ‘to view the tiny,” because microscopes are used to study things that are too small to be easily observed by other methods. The type of microscope that we will be using in this lab is a compound light microscope. Light microscopes magnify the image of the specimen using light and lenses. The term compound means that this microscope passes light through the specimen and then through two different lenses. The lens closest to the specimen is called the objective lens, while the lens nearest io the user's eye is called the ocular lens or eyepiece. When you use a compound light microscope, the specimen being studied is placed on a glass slide. The slide may be either a prepared slide that is permanent and was purchased from a science supply company, or it may be a wet mount that is made for temporary use and is made in the lab room. Objectives: In this lab you will: . Learn the parts of a compound light microscope and their functions. Learn how to calculate the magnification of a compound light microscope. Learn how to make a wet mount slide. Understand how the orientation and movement of the specimen’s image changes when viewed though a campound light microscope. Learn the proper use of the low and high power objective lenses. Learn the proper use of the coarse and fine adjustments for focusing. On BOs Materials: microscope water slides. pipette cover slips scissors lens paper magazine picture with various colors paper towels hairs of different color sheets of newspaper/phonebook other objects for viewing Procedure: Part |. Learning about the microscope 1. One member of your fab group should go and get a microscope. Always carry the microscope in an upright position (not tilted) using two hands. One hand should hold the micrascope’s arm and the other hand should support the base, as shown in Figure 1. Set it down away from the edge of the table. Aways remember that a microscope is an expensive, precision instrument that should be handled carefully. 2. Plug the microscope in at your lab desk. Turn it on and make sure that the light comes on (it may take a second or two to warm up). If the microscope light does not turn on, check with your teacher. 3. Compare your microscope with Figure 2 on the next page. Identify the paris on your microscope and determine the function of each part. 
