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Water Potential: Understanding the Movement of Water in Solutions, Study notes of Chemistry

The concept of water potential, a measure of water's tendency to move from one area to another. It covers the differences in water potential between hypotonic and hypertonic solutions, the role of solute potential and pressure potential, and how to calculate the water potential of a solution using the given formula. The document also includes examples of calculating solute potential for sucrose and nacl solutions.

Typology: Study notes

2021/2022

Uploaded on 09/27/2022

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Water Potential of a Solution
What we already know...
Osmosis will occur across a semi-permeable membrane
to balance the concentrations of two solutions
Direction of water movement:
hypotonic hypertonic
low [solute];
high [free water]
high [solute];
low [free water]
Water Potential (Ψ):
A measure of water’s tendency to move
from one area to another
For movement of water to occur, there must be a
difference in water potential between two areas.
“psi” Ψ = Ψs+ Ψp
For a cell,
Solute potential (Ψs)
measure of the solute
content
always zero for pure water
adding solutes lowers
solute potential
value is always negative
Pressure potential (Ψp)
the pressure a closed container or
a cell wall exerts on its contents
value is usually positive
The rigid cell wall of
a plant exerts an
inward pressure on
its contents
pf3
pf4

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Water Potential of a Solution

What we already know...^ •

Osmosis will occur across a semi-permeable membraneto balance the concentrations of two solutions

Direction of water movement:

hypotonic

hypertonic

low [solute]; high [free water]

high [solute]; low [free water]

Water Potential (Ψ)

A measure of water’s tendency to move

from one area to another

For movement of water to occur, there must be a difference

in water potential between two areas.

“psi”

s

p

For a cell,

Solute potential

s

-^

measure of the solutecontent

-^

always

zero

for pure water

-^

adding solutes

lowers

solute potential

-^

value is always negative

Pressure potential (Ψ

p

-^

the pressure a

closed container

or

a

cell wall

exerts on its contents

-^

value is usually positive

The rigid cell wall of

a plant exerts an inward pressure on

its contents

Example

The solute potential of a cell is -400 kPa. The pressure

potential of the same cell is 100 kPa. What is the waterpotential (Ψ

cell

)of the cell?

Ψ =

Ψ

s

+

Ψ

p

Water flows from areas of

high

water potential

to low

water potential.

Side A Ψ

s^ = - 450 kPa Ψ

p^ = 150 kPa

Side B Ψ

s^ = - 800 kPa Ψ

p^ = 300 kPa

a)

Calculate Ψ for each side

b)

Which way will water move?

Ψ = - 300 kPa

Ψ = - 500 kPa

H

O 2

Side C Ψ

s^ = - 430 kPa Ψ

p^ = 200 kPa

Side D Ψ

s^ = - 100 kPa Ψ

p^

= 50 kPa

Side G Ψ

s^

= - 200 kPa Ψ

p^

= 50 kPa

Side H Ψ

s^

= - 500 kPa Ψ

p^ = 200 kPa

Side E Ψ

s^

= - 500 kPa Ψ

p^ = 200 kPa

Side F

Ψ

s^ = - 400 kPa Ψ

p^ = 100 kPa

Try these....

Example 3 A sample of root tissue is found to have a water potential of

Ψ = - 2.1 bars. The tissue is placed in an open beaker of0.5 M sucrose solution at a temperature of 25°C.

Ψ

s

= - i

R

C

T

R = 0.0831 L•bar/mol•K

a)

What is the pressure potential ofthe sucrose solution?

b)

Determine the solute potential ofthe sucrose solution.

c)

In which direction –

into root or

out of root

  • will water flow?

Tying it all together...^ •

Water moves from areas of higher waterpotential to lower water potential.

▫^

Since water potentials are usually negative, thistranslates to: less negative to more negative.

Water potential can be altered by changing thesolute potential and/or pressure potential.

Ψ

=

Ψ

s

+

Ψ

p

Ψ

s

= - i R C T

= zero for pure water

zero in an open

container or animal cell

The trickiest part is often keeping this terminology straight!!

hypotonic

hypertonic

low [solute]; high [free water]

high [solute]; low [free water]

high water potential

low water potential

HIGH

HIGH

LOW

LOW