PRE-LAB FOR YEAST RESPIRATION AND
FERMENTATION
PURPOSE:
To identify the products of yeast cultures grown under aerobic and anaerobic
conditions
STUDENTS' ENTERING COMPETENCIES:
Before doing this lab, students should understand:
Safety
The term: Cellular Respiration
The products of yeast cultures grown under aerobic and anaerobic
conditions
Differences between cellular respiration and fermentation
Distillation
Yeast cultures
Principles of Gas Chromatography
How to use an analytical balance
Indicators
How to use a centrifuge
TOPS YEAST EXPERIMENT EXTENSION ACTIVITIES
1. Yeast/H2O2 with watch glass and glowing splint
2. Bread/pretzel making
3. Taste test: matzo bread
4. Yeast and sugar in Erlenmeyer flask will blow up balloon over mouth of flask
5. Yeast reproduction: budding
6. Use straw to expire into bromthymol blue
7. Indicators and pH
8. Use centrifuge to separate oil, water, and food coloring
9. Use microscope to observe/identify yeast cells
10. Graphing activity: put 5 mL of EtOH and 3 mL of H2O in a 25 mm diameter test
tube. Place a #4, two hole rubber stopper in the test tube with a thermometer in
one hole and a glass bend in the other. Slowly boil. Record temperature at 3
seconds intervals. Graphing of data will display plateaus of boiling points of
liquids.
REAL LIFE EXAMPLES
1. STEROID TESTING: - Ben Johnson, a member of the Canadian Olympic track
team, was disqualified based on
positive test results for anabolic steroid use as
concluded by the use of gas chromatography.
