Human Hematopoietic Stem Cell
CD34
CD38low/neg
CD59
CD90 (Thy1)
CD117 (c-kit)
CD133
CD135 (Flt3)
CD164
CD338
linneg
GaTa2
TdT
Multi-Potent Progenitor (MPP)
CD34
CD135 (Flt3)
linneg
TdT
Common Lymphoid Progenitor (CLP)
Pro-B
CD10
CD19
CD20
CD22
CD34neg
CD72
CD74
CD124
CD164
CD252
CD268
CD275
HLa-DR
Pre-NK/T
CD5
CD34
Pro-NK
CD7
CD10neg
CD34neg
CD117 (c-kit)
Immature NK
CD34neg
CD94neg
CD117 (c-kit)
CD122
CD161
Ets-1
Id2
Id3
NKT
CD3
CD8
CD16
CD56
CD69
CD161
Vα24Jα18TCR
Id2
PLZF
CD4 ISP (Immature
Single Positive)
CD1a
CD2
CD3
CD4
CD5
CD7
CD34
CD45RO
CD127 (IL-7Rα)
TCRβlow
Early Double
Positive (EDP)
CD1a
CD2
CD3
CD4
CD5
CD7
CD8α
CD38
CD45RO
CD127 (IL-7Rα)
TCRβ
Double Positive (DP)
γδ T
CD4neg
γδ TCR
Sox13
CD8
CD8
Granulysin
Granzyme a
Granzyme B
Perforin
Runx3
CD4
CD4
Pre-B
CD10
CD19
CD20
CD22
CD34neg
CD72
CD74
CD79a
CD124
CD164
CD252
CD268
CD275
HLa-DR
IgM
Pax5
Immature B
CD5
CD10
CD19
CD20
CD21
CD22
CD23
CD24
CD32
CD37
CD40
CD48
CD52
CD72
CD75
CD79a
CD79b
CD80act
CD84
CD86act
CD98
CD124
CD137 (41BB)act
CD152act
CD153
CD180
CD184
CD185
CD196
CD200
CD210
CD229
CD252act
CD253
CD267 (TaCI)
CD275
CD279 (PD-1)
CD307
CD317 (BST2)
CD319 (CRaCC)
IgD
IgG
IgM
Pre-T
CD1a
CD2
CD3
CD5
CD7high
CD28
CD34
CD45
CD127 (IL-7Rα)
NOTCH1
Mature B
CD5
CD19
CD20
CD21
CD22
CD23act
CD24
CD27act
CD32
CD35
CD37
CD40
CD48act
CD49b
CD49c
CD49d
CD52
CD72
CD75
CD79a
CD79b
CD80act
CD84
CD86act
CD98
CD124
CD137 (41BB)act
CD152 (CTLa-4)
act
CD180
CD184 (CXCR4)
CD185 (CXCR5)
CD192 (CCR2)
act
CD196 (CCR6)
CD200
CD210
CD229
CD252act
CD253act
CD267 (TaCI)
CD268 (BaFFR)
CD269 (BCMa)
CD272
CD275
CD279 (PD-1)act
CD307
CD317 (BST2)
CD319 (CRaCC)
CD360 (IL-21R)
HLa-DR
IgD
IgG
IgM
Blimp-1
Bcl-6
Pax5
Plasma
Common Myeloid Progenitor (CMP)
CD33
CD34
CD45Ra
CD117 (c-kit)
CD123low
CD131
CD164
CD173
CD174
Ikaros
PU.1
Megakaryocyte-
Erythroid
Progenitor (MEP)
Granulocyte-Myeloid
Progenitor (GMP)
CD34
CD45Ra
CD123
PU.1
CD33
CD34
CD36
CD 110
CD123high
CD131
CD16 4
FOG-1
GaTa1
GaTa 2
Macrophage
CD 11a
CD 11c
CD14
CD16
CD19
CD23
CD26
CD31
CD32
CD36
CD40
CD45RO
CD52
CD63
CD68
CD71
CD74
CD87
CD88
CD92
CD 119
CD121b
CD123
CD155
CD156 a
CD163
CD169
CD170
CD19 5
CD204
CD206
CD210
CD280
CD282 (TLR2)
CD284 (TLR4)
CD312
CD354 (Trem-1)
CD357 (GITR)
HLa-DR
Trem-2
PU.1
Conventional DC Precursor
CD11c
CD14neg
CD33dim
Common DC Progenitor
CD11c
CD33dim
Plasmacytoid
DC (pDC)
Conventional
DC (cDC)
Monocyte
Mature NK
CD2
CD7
CD8
CD11b
CD15s
CD16
CD27
CD32
CD43
CD45RC
CD49d
CD56
CD57
CD62L
CD66a
CD81
CD85d (ILT4)
CD85j (ILT2)
CD87
CD94
CD96
CD99R
CD119
CD122
CD132
CD158a
CD158b
CD158f
CD159a (NKG2a)
CD159c (NKG2C)
CD160
CD161
CD162R
CD178
CD181 (CXCR1)
CD182 (CXCR2)
CD183 (CXCR3)
CD198
CD210
CD212
CD218a
CD218b
CD223
CD226
CD244
CD245
CD247
CD253
CD277
CD300a
CD305
CD319 (CRaCC)
CD328 (Siglec-7)
CD335 (NKp46)
CD336
CD337
Granzyme a
Granzyme B
Perforin
Neutrophil Eosinophil
Treg
CD25
CD39
CD73
CD101
CD127low
CD152 (CTLa-4)
CD183 (CXCR3)
CD194 (CCR4)
CD199 (CCR9)
CD357 (GITR)
GaRPact
Foxp3
Helios
Th17
CD161
CD121a (IL-1R1)
CD196 (CCR6)
CD360 (IL-21R)
IL-23R
BaTF
IRF4
RORγt
Th1
CD26
CD94
CD178 (FaSL)
CD195
CD212 (IL-12RB2)
CD218 (IL-18R)
CD254 (TRaNCE)
CD278 (ICOS)
NOTCH3
Tim-3
T-bet
Th2
CD30
CD124 (IL-4R)
CD184 (CXCR4)
CD198 (CCR8)
IL-17RB
NOTCH1
NOTCH2
Tim-1
GaTa3
Th9
CD124 (IL-4R)
IL-17RB
IRF4
Th22
CD140 (PDGFR)
CCR10
Tfh
CD84
CD126 (IL-6R)
CD185 (CXCR5)
CD278 (ICOS)
CD360 (IL-21R)
Bcl-6
Mast Basophil
CD9
CD11a
CD11b
CD13
CD16
CD17
CD25
CD32
CD33
CD38
CD43
CD88
CD123
CD125
CD218 (IL-18R)
CD154
CD192
CD193
CD203c
CD281 (TLR1)
CD282 (TLR2)
CD284 (TLR4)
CD286 (TLR6)
CD289 (TLR9)
CD294
FcεR1
IgE
C/EBPα
GaTa2
Megakaryocyte
CD41
CD42a
CD42b
CD42c
CD42d
CD49f
CD51
CD61
CD110
CD112
CD123
Platelet
CD9
CD17
CD23
CD31
CD36
CD41
CD42a
CD42b
CD42c
CD42d
CD49b
CD49f
CD51
CD60a
CD61
CD84
CD92
CD110
CD147
CD151
CD173
CD226
GaRP
Activated Platelets
CD54
CD62p
CD63
CD107a
CD107b
CD109
Erythrocyte
CD35
CD44
CD123
CD173
CD233
CD234
CD235a
CD235b
CD236
CD236R
CD238
CD239
CD240
CD241
CD242
General T Cell Markers
CD2
CD3
CD5
CD6
CD7
CD27
CD28
CD30
CD35
CD40
CD43
CD45Ra
CD45RB
CD45RC
CD52
CD57
CD60a
CD60b
CD60c
CD62L
CD75s
CD81
CD84
CD85j (ILT2)
CD89
CD94
CD98
CD99R
CD101
CD103
CD107aact
CD107bact
CD 119
CD121b
CD122
CD123
CD127
CD130
CD132
CD150
CD158 a
CD158 b
CD158 f
CD159a
CD16 0
CD161
CD16 8
CD171
CD172a (SIRPα)
CD181 (CXCR1)
CD182 (CXCR2)
CD184 (CXCR4)
CD191 (CCR1)
CD192 (CCR2)
CD193 (CCR3)
CD195 (CCR5)
CD196 (CCR6)
CD197 (CCR7)
CD198 (CCR8)
CD199 (CCR9)
CD210
CDv218 a
CD218b
CD226
CD229
CD244
CD247
CD277
CD279
CD300a
CD300c
CD305
CD316
CD317 (BST2)
CD319 (CR aCC)
αβ TCR
CD4
low
CD9
CD 11b
CD 11c
CD13
CD14
CD15
CD17
CD28
CD31
CD32
CD33
CD35
CD36
CD38
CD40
CD45RO
CD45Ra
CD45RB
CD49b
CD49e
CD49f
CD63
CD64
CD65s
CD68
CD74
CD84
CD85a (ILT5)
CD85d (ILT4)
CD85h (ILT1)
CD85j (ILT2)
CD85k (ILT3)
CD86
CD87
CD89
CD91
CD92
CD93
CD101
CD 111
CD 112
CD 115
CD 116
CD 119
CD121b
CD123
CD131
CD14 2
CD147
CD155
CD156 a
CD157
CD162
CD163
CD16 8
CD170
CD171
CD172a (SIRPα)
CD172b
CD18 0
CD18 4 (C XCR4)
CD191 (CCR1)
CD192 (CCR2)
CD19 5 (CCR5)
CD19 8 (CCR 8)
CD210 (IL-10R)
CD213a1 (IL-13Rα1)
CD213a2 (IL-13Rα2)
CD226
CD277
CD281 (TLR1)
CD282 (TLR2)
CD284 (TLR4)
CD300a
CD300c
CD300e (IREM-2)
CD300f (IREM-1)
CD302
CD305
CD312
CD317 (BST2)
CD322
CD329
GITRL
PU.1
CD10
CD15
CD16b
CD17
CD24
CD32
CD35
CD43
CD66a
CD66b
CD66d
CD89
CD93
CD 112
CD 114
CD 116
CD157
CD177
CD181 (CXCR1)
CD281 (TLR1)
CD282 (TLR2)
CD284 (TLR4)
CD286 (TLR6)
CD289 (TLR9)
MPO
Trem-1
CD1c
CD1d
CD2
CD4low
CD 11b
CD 11c
CD13
CD14 neg
CD33
CD45RO
CD64
CD68
CD80
CD85j (ILT2)
CD85k (ILT3)
CD86
CD105
CD 112
CD123neg
CD141low
CD163
CD172a (SIRPα)
CD19 5 (CCR5)
CD197 (CCR7 )
CD205
CD282 (TLR2)
CD283 ( TLR3)
CD284 (TLR4)
CD286 (TLR6)
CD300e
CLEC-9a
HLa-DR
CD4
CD 11c neg
CD45Ra
CD49d
CD85g (ILT7)
CD85k (ILT3)
CD123
CD282 (TLR2)neg
CD284 (TLR4)neg
CD289 (TLR9)
CD303 (BDC a-2)
CD304 (BDCa-4)
HLa-DR
TLR7
Spi-B
CD9
CD 11b
CD15
CD24
CD32
CD35
CD43
CD64
CD 116
CD117 (c-kit)
CD123
CD125
CD126
CD181 (CXCR1)
CD244
FcεR1
IgE
CD9
CD 11b
CD15
CD24
CD32
CD35
CD64
CD244
CD 116
CD123
CD125
CD126
FcεR1
IgE
––
= Proposed Pathway
act = Expressed On activated
low = Low Expresion
high = High Expresion
neg = Negative
= Intracellular antigen
= Subset
= Pan Marker/Common
Defining Marker
LEGEND
LYMPHOID MYELOID
CD117 (c-kit)low
CD127
CD135 (Flt3)
CD164
HLa-DR
aiolos
c-mybhigh
C/EBPαneg
GaTa2neg
GaTa3
PU.1high
TdT
CD7
CD10
CD24neg
CD34
CD38
CD45Ra
CD90low
CD133
CD138
CD183 (CXCR3)
CD184 (CXCR4)
CD269 (BCMa)
aiolos
Blimp-1
IRF4
XBP-1
CD19neg
CD20neg
CD27
CD252
CD52neg
CD126
CD38
CD72neg
CD79a
CD1a
CD2
CD3
CD4
CD5
CD7
CD8α
CD8β
CD45RO
CD127 (IL-7Rα)
TCRβ
ThPOK
TOX
Differentiation of the Human Hematopoietic Stem Cell
Human hematopoietic development from the hematopoietic
stem cell to the end st age cells illustrating marker s found at
each stage as de termined through the 9th HL DA congress.
Q312061 Hematopoietic Stem Cells 0912
www.eBioscience.com
©2012 eBioscience, Inc. An Affymetrix Company. All rights reserved.
Customers in countries where direct
sales are not available may contact
their eBioscience distributor listed at
www.eBioscience.com/distributors
Differentiation of the Human Hematopoietic Stem Cell
NORTH AMERICA: CUSTOMER SERVICE 888.999.1371 • info@eBioscience.com | TECHNICAL SUPPORT 888.810.6168 • tech@eBioscience.com
EUROPE: CUSTOMER SERVICE +43 1 796 40 40-304 • europe@eBioscience.com | TECHNICAL SUPPORT +43 1 796 40 40-120 • tech@eBioscience.com
